- What are the definitions of the following with respect to DNA mutations
- Point mutation
- What are the definitions of the following DNA mutations with respect to the protein produced?
- Missense mutation
- Nonsense mutation
- Silent mutation
- Frame shift mutation
- Using the codon table below answer the following questions:
You have the following wild type coding gene sequence:
In numbers below represent the base number of the sequence.
|mutation||Gene sequence||Protein sequence||Type of mutation||Type of protein mutation|
|Wild type (wt)||ATG AAG CTA ATA TCG TAA GGG||wt||wt|
|A4T (A at position 4 is a T)|
|C inserted at position 4|
|Delete bases 4-6|
- What is the difference between a spontaneous mutation and a mutation generated by a mutagen?
- What are the ways that DNA bases can become damages during normal cell processes?
- For each of the following DNA repair mechanisms, explain what is repaired, and how the repair mechanism works:
Repair replication errors:
Repair UV damage:
Replace damaged nitrogenous bases:
Repair DS DNA breaks (2 different ways)
- What is SOS repair and why is it induced? What is the advantage and disadvantage of this pathway?
- A suspected chemical mutagens (compound X and compound Y) are screened by the Ames test. Which should be considered mutagens? Explain your conclusion.
Note: These are Petri plates and the yellow dots represent colonies.
- What are 5 methods that bacteria use to transfer DNA BETWEEN cells? Briefly explain each.
- What are transposons? What are the basic elements that make up a transposon?
- What are genomic islands, and how can they be identified?
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