Serology (v.1) Date ………….
Name Group
1. ……………………………………… ……………..
2. ……………………………………… ……………..
3. ……………………………………… ………………
Reagents for forward (cell) typing (blood group test):
- Patient’s red blood cells (RBC) (10%) in 0.9% NaCl
- monoclonal antibodies: anti-A, anti-B, anti-D
- serological plate (slide with wells)
Reagents for reverse (serum) typing (detection of antibodies in the serum)
- patient’s serum
- standard red blood cells: A, B, O
- serological plate (slide with wells)
Preparation of blood group test:
Place 1 drop of anti-A and 1 drop of anti-B and 1 drop of anti-D reagent (antiserum) separately on a labeled slide.
Add 1 drop of patient’s RBC to each drop of the typing antiserum.
Mix the cells and reagent using a clean stick, spread each mixture evenly in whole well.
Tilt the slide and leave the test for 2 minutes at room temperature, then rock again and look for agglutination.
Record the results filling the table by placing “+” if the agglutination took place and “-“ if there was no agglutination.
According to detected blood group fill the table placing “+” or “-“ in right place!!!
| Tested red blood cells | Monoclonal antibodies (antiserum reagents) | Detected antigens on red blood cells = detected blood group | ||
| anti-A | anti-B | anti-D | ||
| Patient 1 | ORh- | |||
| Patient 2 | A1Rh- |
Preparation of reverse typing test (detection of antibodies in patient’s serum):
Place 1 drop of standard RBC A and 1 drop of standard RBC B and 1 drop of standard RBC O separately on a labeled slide.
Add 1 drop of patient’s serum to each drop of the typing standard RBC’s.
Mix the cells and reagent using a clean stick, spread each mixture evenly in whole well.
Tilt the slide and leave the test for 2 minutes at room temperature, then rock again and look for agglutination.
Record the results filling the table by placing “+” if the agglutination took place and “-“ if there was no agglutination.
According to detected blood group, what antibodies we expect to detect in the sera of the above patients – fill the table placing “+” or “-“ in right place and write the names of the detected antibodies!!!
| Tested serum | Standard red blood cells (RBC) | Detected antibodies in serum | ||
| A | B | O | ||
| Patient 1 | ||||
| Patient 2 |
Answer the questions:
1. Cross-match test. In normal situation transfusion can be done when:
a. donator RBCs react with receiver serum
b. donator RBCs do not react with receiver serum
c. donator serum react with receiver RBCs
d. all answers are correct
2. List 6 serology tasks:
a.
b.
c.
d.
e.
f.
3. List transfusion complications with a little details for each:
a.
b.
c.
d.
e.
4. List clinically significant blood group systems:
a.
b.
c.
d.
e.
f.
