Data Sheet: Activity – Biomolecules
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Activity Data Code SCJ
Procedure I – Group 1: Known Sample Solutions
Complete the table below using your data from Procedure I. For each sample solution (A-E) indicate any color change that occurs when a test solution is added. Fill in the last column (Biomolecule Classification) using data found under the Activity Form tab.
Data Table I
| Sample Solution | Test Solution 1 Reaction | Test Solution 2 Reaction | Test Solution 3 Reaction | Test Solution 4 Reaction | Test Solution 5 Reaction | Biomolecule Classification |
| A | red | sugar | ||||
| B | red | blue | starch | |||
| C | green | fat | ||||
| D | purple | protein | ||||
| E | purple | grey | amino acid |
Observations and Questions
[1] Write a hypothesis statement for the experiment you conducted for Procedure I.
[2] Which variable represents the independent variable? What information helped you to come to that decision?
[3] Would you characterize this experiment as qualitative or quantitative? What information helped you to come to that decision?
[4] Refer to your completed Data Table I and place the appropriate answer to each of the statements below.
Answers: amino acid, starch, fat, sugar or starch, protein or amino acid
Statements:
Test Solution 1 reacts with:
Test Solution 2 reacts with:
Test Solution 3 reacts with:
Test Solution 4 reacts with:
Test Solution 5 reacts with:
Procedure II – Group 2: Unknown Sample Solutions
Complete the table below using your data from Procedure II. For each sample solution (A-E) indicate any color change that occurs when a test solution is added. Next, use Data Table I to determine the biomolecule classification for each sample solution. Hint: compare the color changes in both tables to make your determinations.
Data Table II
| Sample Solution | Test Solution 1 Reaction | Test Solution 2 Reaction | Test Solution 3 Reaction | Test Solution 4 Reaction | Test Solution 5 Reaction | Biomolecule Classification |
| A | ||||||
| B | ||||||
| C | ||||||
| D | ||||||
| E |
Observations and Questions
[5] In Procedure II, did any of your unknown sample solutions (A, B, C, D, E) contain sugars? If yes, which one? What information helped you to come to that decision?
[6] In Procedure II, did any of your unknown sample solutions (A, B, C, D, E) contain protein? If yes, which one? What information helped you to come to that decision?
[7] Using the laboratory tests in this experiment, would you be able to distinguish between a small molecule such as amino acid from a larger molecule such as a protein? Explain why or why not.
[8] Using the laboratory tests in this experiment, would you be able to distinguish between a starch and a sugar such as glucose? Explain why or why not.
